EnergyMitochondrial HealthCellular RepairHealthy AgingCellular energy and mitochondrial support

RESEARCH REFERENCE

NAD+

Essential coenzyme for cellular energy production, DNA repair, and sirtuin activity that declines with age.

Quick Reference

Dose: SC 50–200 mg; higher for IV
Frequency / schedule: 2–3× weekly (SC) or periodic higher-dose sessions
Route: Subcutaneous (most practical for self-administration)
Timing: Morning preferred
Fasting: Not strictly required but morning fasted is common
Cycle: 8–12 weeks or ongoing
Time off: Optional 2–4 week breaks
Common vial strengths: 100 mg, 500 mg, 1000 mg

How this works

NAD+ is a critical cofactor for hundreds of enzymatic reactions, including those involving sirtuins (longevity-related deacetylases), PARPs (DNA repair), and mitochondrial energy metabolism. Levels decline with age. Direct subcutaneous or intravenous administration raises NAD+ levels, while oral precursors (NMN/NR) increase it more gradually via the salvage pathway.

Benefits + side effects

Potential Benefits

Subjective improvements in energy, recovery, and mental clarity (commonly reported)
Supports mitochondrial and cellular repair processes

Side Effects

Flushing, chest tightness, or nausea (more common with rapid IV)
Mild with subcutaneous use
Generally well tolerated at research doses

Dosing / Protocol

Reference Protocol

Phase / week	Dose	Frequency	Timing	Notes
Standard SC	100 mg	2–3× weekly	Morning	Most practical self-admin
Higher SC	150–200 mg	2–3× weekly	Morning	If well tolerated
Loading style	50 → 100 mg	2× weekly stepped	Morning	Conservative start

Timing + Fasting Notes

Morning is preferred (energy-supporting). Can be taken with or without food, though many prefer fasted or lightly fasted.

Reconstitution

Common vial strengths100 mg, 500 mg, 1000 mg

Approved preparation example
500 mg + 5 mL BAC → 100 mg/mL (100 mg = 1 mL / 100 units)

Practical Reference

Storage: Lyophilized frozen/refrigerated; reconstituted refrigerated, use within 28 days
Route / injection context: Subcutaneous (most practical for self-administration)
Bloodwork / markers: Subjective energy; NAD metabolites if available
Common vial strengths: 100 mg, 500 mg, 1000 mg
Cycle / time off: 8–12 weeks or ongoing · Optional 2–4 week breaks
Practical notes: Research peptide — reconstitute yourself. Many stack with oral NMN/NR for maintenance between injections. Research use only.

References

Tracing the Metabolic Flux of Orally Administered NAD+ClinicalTrials.gov · 2024

CLINICAL TRIAL

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